Table 1. P. falciparum Strains and Experimental Design Used in This Study.
| Purpose | Strain & sub-line | Description | Method of cDNA/gDNA preparation | No. of cycles in culture | No. of replicate hybridizations |
| Testing amplification method | |||||
| a3D7 | Laboratory | Unamplified | Many | 2 | |
| Transcriptome analysis | |||||
| 3D7 | Laboratory | Amplified | Many | 2 | |
| bDd2 | Laboratory | Amplified | Many | 1 | |
| cT9/96 | Laboratory | Amplified | Many | 1 | |
| dP1 | Field, severe | Amplified | 62 | 3 | |
| dP3 | Field, severe | Amplified | 63 | 3 | |
| dP4 | Field, mild | Amplified | 48 | 3 | |
| dP5 | Field, mild | Amplified | 66 | 2 | |
| dP6 | Field, mild | Amplified | 59 | 3 | |
| Gene copy number analysis | |||||
| dP1.A | As above | Unamplified | e+21 | 3 | |
| dP3.A | Unamplified | +24 | 3 | ||
| dP4.A | Unamplified | +31 | 3 | ||
| dP5.A | Unamplified | +21 | 3 | ||
| dP6.A | Unamplified | +23 | 3 | ||
| dP1.B | Unamplified | +8 | 1 | ||
| dP4.B | Unamplified | +14 | 1 | ||
| dP6.B | Unamplified | +8 | 1 | ||
a
Derived from a patient in the Netherlands living near the airport [131]. Believed to be of African origin.
b
Obtained by cloning of strain W2-mef, a clone of Indochina III/CDC that had been under long-term mefloquine pressure [132].
d
Obtained from Kilifi, Kenya, from patients admitted to the hospital with severe or mild malaria.
e
The “+” indicates the number of cycles of culture in addition to those in the top part of the table.