Fig. 1.

SELEX selection on recombinant DBL1α^His. A 2´-F substituted RNA library of 10¹⁴ sequences was incubated with 0.45–1.3 µM recombinant DBL1α^His bound to Ni-NTA beads. 2′F-RNA was internally labelled with ³²P-[αATP] and the percentage of bound 2′F-RNA recovery was quantified using scintillation count of obtained fractions and listed for each of the eight selection rounds. White bars represent pre-selection on the 100 µl Ni-NTA matrix. Black bars are selection on Ni-NTA with bound DBL1α^His