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. 2009 Oct 21;97(8):2277–2286. doi: 10.1016/j.bpj.2009.07.041

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Western blotting studies on murine gastrocnemius with the PEVK-specific anti-titin antibody 9D10. (Left panel) Typical Western blot result with 9D10 labeling and PonceauS (PonS) staining of total titin. Note the stronger 9D10 labeling in neonatal (d5) versus adult gastrocnemius. (Right panel) Quantitative analysis of 9D10 labeling (normalized to protein levels obtained from the PonceauS membrane) in neonates as a percentage of labeling in adults. ^∗Significantly different from adult, p < 0.05.