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. 2009 May 26;72(6):1517–1529. doi: 10.1111/j.1365-2958.2009.06740.x

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Journal compilation © 2009 Blackwell Publishing

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Fig. 3 — Purification and characterization of BB0426_opt. A. Commassie-stained SDS-PAGE gel with (1) E. coli lysate expressing BB0426_opt and eluted fraction from a T7 affinity column. B. HPLC chromatograms showing the enzymatic activity of BB0426_opt. In this assay, deoxyinosine (dIno) served as the deoxyribosyl donor and adenine (Ade) the deoxyribosyl acceptor: deoxyadenosine (dAdo) and hypoxanthine (Hyp) are the products of the reaction.