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. 2009 May 26;72(6):1517–1529. doi: 10.1111/j.1365-2958.2009.06740.x

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Journal compilation © 2009 Blackwell Publishing

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Fig. 4 — [³H]-Hyp conversion and incorporation into nucleic acids. A. [³H]-Hyp conversion and incorporation into the RNA and DNA of strains B31A3 68-1, ΔguaAB, ΔguaAB/pBSV2G (vector control) and ΔguaAB/pBSV2G guaAB (complement). Femtomoles of [³H]-Hyp incorporated were calculated from the counts per minute (cpm) and the specific activity of the labelled substrate. Error bars represent the standard deviations of triplicate samples. B. HPLC analyses of the incorporation of [³H] into individual deoxynucleosides of DNA isolated from [³H]-Hyp labelled B. burgdorferi cells. The left side of the z-axis, [³H]-Hyp was determined to be incorporated primarily as dGuo. The right of the z-axis shows the per cent composition of unlabelled DNA as a control to verify that DNA digestion went to completion.