Table 1.

Specific activities of BB0426_opt with various substrates compared with DRTase I^a and DRTase II^a from Lactobacillus helveticus (Kaminski, 2002).

			Specific activity (nmol min−1 mg−1)
			B. burgdorferi	L. helveticus
Deoxyribose donor	Acceptor	Enzymatic product	BB0426	DRTase I	DRTase II
Purines
Deoxyinosine	Adenine	Deoxyadenosine	42 ± 1.2b	83	3
Deoxyinosine	Guanine	Deoxyguanosine	45 ± 4.9	–	–
Deoxyadenosine	Hypoxanthine	Deoxyinosine	16 ± 2.5	61	8
Deoxyadenosine	Guanine	Deoxyguanosine	94 ± 2.4	–	–
Deoxyguanosine	Adenine	Deoxyadenosine	85 ± 10.2	50	16
Deoxyguanosine	Hypoxanthine	Deoxyinosine	18 ± 0.7	54	3
Deoxyadenosine monophosphate	Hypoxanthine	Deoxyinosine	< 1	–	–
Deoxyguanosine monophosphate	Hypoxanthine	Deoxyinosine	< 1	–	–
Pyrimidines
Deoxythymidine	Cytosine	Deoxycytosine	< 1	< 1	28
Deoxycytosine	Thymine	Deoxythymidine	2	< 1	32
Purine/pyrimidine
Deoxyinosine	Cytosine	Deoxycytosine	< 1	< 1	8
Deoxyadenosine	Cytosine	Deoxycytosine	< 1	< 1	34

^aDRTase I or II: deoxyribosyltransferase class I or II.

^bMean values and standard deviations represent triplicate analyses.