Table 3.

B. burgdorferi strains and primers used in this study.

Clone	Genotype	Plasmids lost	Reference
B. hermsii DAH	Wild-type	Unknown	Hinnebusch et al. (1998)
B31A3 68-1	Parent strain	cp9, lp25, lp56	This study
B31A3 m9	Parent strain	cp9, lp21	Tilly et al. (2004)
guaA::kan	A3 M9 guaA:: flgBp-kan	cp9, lp21	This study
ΔguaAB	A3 68-1 guaAB::flaBp-aadA	cp9, lp25, lp56	This study
ΔguaAB/pBSV2G	A3 68-1 guaAB::flaBp-aadA	cp9, lp25, lp56	This study
ΔguaAB/pBSV2G guaAB	A3 68-1 guaAB::flaBp-aadA/ pBSV2G guaAB	cp9, lp25, lp56	This study
Number	Primer name	Sequence
p1	guaB−500	5′-TGCTGATCCTATGACGTTCTTAAGC-3′
p2	guaA+500	5′-TAGGGTTGATATTGCATAAGCTCCCGC-3′
p3	guaB 13852-SalI 3′	5′-GCGTCGACAAAAGATCAAAATATTGCATCCTTC-3′
p4	guaA 16727-SalI 5′	5′-GCGTCGACAGCATATTTGGCTTTGCTTATGTCG-3′
p5	flaB-XhoI 5′	5′-CCGCTCGAGCTGTCGCCTCTTGTGGCTTC-3′
p6	aadA-XhoI 3′	5′-CCGCTCGAGTTATTTGCCGACCTACCTTGG-3′
p7	guaAcompF-SalI	5′-GCGTCGACGGTTTATAGCTAGATCTTTTGATTTGGC-3′
p8	guaAcompRC-SalI	5′-GCGTCGACTCAGCAGAATTTGCAGATGTATTCCC-3′
p9	guaA F	5′-GATTTTGGATCCCAATATAGCC-3′
p10	guaA R	5′-CCCATTCTATGGTTGATGGAGGCTTAGA-3′
p11	kan 5′-NdeI	5′-CATATGAGCCATATTCAACGGGAAACG-3′
p12	kan term XbaI	5′-GCTCTAGACTAGCGCCGTCCCGTCAA-3′
p13	guaA16159-SalIF	5′-GTCGACGGATGGAATTGTAGGCCG-3′
p14	guaA15926-SalIRC	5′-GTCGACGTAAACACTGGATTGTTGCGC-3′
p15	flgBPo-XhoI	5′-TAATACTCGAGCTTCAAGGAAGATTT-3′
p16	aacC1-NheI	5′-GCTAGCCGATCTCGGCTTGAACG-3′