Fig. 7.

Transmission electron microscopy (TEM) images of wild-type 10403S and 10403SΔlmo0927 L. monocytogenes strains. Overnight cultures of wild-type 10403S (WT) and 10403SΔlmo927 (Δ0927) strains were back-diluted and grown for the indicated time at 30°C or 37°C. Bacteria were fixed and prepared for TEM as described under Experimental procedures and representative images are shown: WT grown for 3.5 h at (A) 30°C and (B) 37°C; Δ0927 grown for 3.5 h at (C) 30°C and (D) 37°C; (E–H) Δ0927 grown for 8.5 h at 37°C. Images were taken at (A–D) 49 000×; (E) 30 000×; (F) 68 000×; (G and H) 98 000× magnification and scale bars are shown.