Table 1.
Summary of TLC and MALDI-TOF data of glycolipids produced by wild-type and mutant L. monocytogenes strains.
| Lipid (fatty acid chain length) Formula – calculated mass | WT | Δ2553 | Δ2554 | Δ2555 | Δ0644 | Δ0927 |
|---|---|---|---|---|---|---|
| Top band | X | X | X | Not observed | X | X |
| Glc-DAG (C17, C15) | ||||||
| C41H78Na1O10 – 753.5 | 753.6 | 753.5 | ||||
| Glc-DAG (C15, C15) | ||||||
| C39H74Na1O10 – 725.5 | 725.4 | |||||
| Middle band | X | X | Not observed | Not observed | X | X |
| Gal-Glc-DAG (C17, C15) | ||||||
| C47H88Na1O15 – 915.6 | 915.7 | 915.6 | 915.6 | 915.7 | ||
| Gal-Glc-DAG (C15, C15) | ||||||
| C45H84Na1O15 – 887.6 | 887.6 | 887.6 | 887.6 | 887.6 | ||
| Gal-Glc-DAG (C17, C17) | ||||||
| C49H92Na1O15 – 943.6 | 943.8 | 943.6 | 943.6 | 943.7 | ||
| Bottom band | X | X | Not observed | Not observed | Not observed | X |
| GroP-Gal-Glc-DAG (C17, C15) | ||||||
| C50H95Na1O20P1 – 1069.6 | 1069.5 | 1069.5 | 1069.8 | |||
| GroP-Gal-Glc-DAG (C17, C15) | ||||||
| C50H94Na2O20P1 – 1091.6 (disodium adduct) | 1091.5 | 1091.5 | 1091.8 | |||
| Ala-GroP-Gal-Glc-DAG (C17, C15) | Not observed | Not observed | Not observed | Not observed | Not observed | X |
| C53H100 N1Na1O21P1 – 1140.6 | 1140.8 | |||||
| Ala-GroP-Gal-Glc-DAG (C17, C15) | ||||||
| C53H99 N1Na2O21P1 – 1162.6 (disodium adduct) | 1162.8 |
Presence or absence of glycolipids in different L. monocytogenes strains shown on top is denoted with ‘X’ when present and ‘Not observed’ when absent. Abbreviations for glycolipids in top, middle and bottom bands as indicated in Fig. 3 are shown in the left column with fatty acid chain length given in parenthesis along with molecular formula and calculated absolute mass of sodium adducts or disodium adducts (minus one proton).