FIG. 1.

Sporangiospores from RPMI 1640 and RPMI 1640 supplemented with iron were statistically significantly more lethal to flies than sporangiospores collected from YAG (P < 0.0001 and 0.0117, respectively). Data from three experiments per strain were pooled to obtain survival curves indicating the lethality of three Cunninghamella strains cultured in poor medium (RPMI 1640), rich medium (YAG), and RPMI 1640 supplemented with iron (RPMI + 10 mcg/ml FeSO4). Data are presented as a Kaplan-Meir plot developed using Prism 4 software (GraphPad Software, Inc., La Jolla, CA), and curves were compared by the log-rank test. Statistical significance was defined by a P value of <0.05.