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Diagram of the nondividing Huh7 cell culture system. To establish nondividing cultures, Huh7 cells are seeded at 80% confluence on collagen-coated plasticware. Upon reaching 90% confluence, media are replaced with complete DMEM supplemented with 1% DMSO, and cultures are incubated for an additional 20 days. Six days after the initiation of DMSO treatment, cultures reach steady-state cell numbers.
