TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and property | Source or reference |
|---|---|---|
| E. coli DH5α | F− φ80dlacZ M15 (lacZYA-argF)U169 recA1 endA1 hsdR17(rK−, mK+) phoA supE44 thi-1 gyrA96 relA1 | Commercial (Invitrogen) |
| S. aureus | ||
| RN4220 | Derived from NCTC8325; restriction deficient | 5 |
| Xen36 | Kanr, derived from ATCC 49525; possesses a stable copy of the modified Photorhabdus luminescens luxABCDE operon at a single integration site on a native plasmid | 7 |
| Xen36 Δdak2 | Transposon insertion mutant in DAK2 family gene | This study |
| Xen36 ΔfecCD | Mutant of Xen36with deletion of fecCD gene, obtained by allelic replacement with pKOR1 | This study |
| Plasmids | ||
| pBT2 | Cmr and Ampr; temperature-sensitive E. coli/Staphylococcus shuttle vector for allelic replacement by homologous recombination | 4 |
| pKOR1 | Cmr and Ampr, temperature-sensitive E. coli/Staphylococcus shuttle vector for allelic replacement by homologous recombination, with att recombination sites for cloning and inducible secY antisense RNA-based counterselection | 2 |
| pEC2 | Ampr and Ermr; contains 1.45-kb ermB fragment of Tn551 (12) | 4 |
| pTX15 | Tetr, contains a xylose-inducible promoter | 21 |
| pBADA7 | Contains the himar1 mariner transposon | 14 |
| pBTn | Used for transposon mutagenesis; contains a 1.45-kb ermB fragment of Tn551 between the himar1 inverted repeats and the himar1 mariner transposon under the control of a xylose-inducible promoter | This study |
| pT181 | Naturally occurring 4.5-kb S. aureus plasmid, Tetr | 11 |
| pT181mcs | pT181 with multiple cloning site of pUC19 (PvuII fragment) cloned in NdeI site | 27 |
| pTdak2 | pT181mcs with dak2-like gene | This study |
| pTfecCD | pT181mcs with fecCD-like gene | This study |