TABLE 3.

Bioluminescence and traditional killing assays with selected mutants

Mutant type and protein encoded by disrupted gene locus	Gene no. in S. aureus Sanger 252	No. of mutations in locus	Difference in killing rate between wild type and mutant determined by:
			Bioluminescence assay (%)	Traditional killing assay (factor)
Mutants with increased susceptibility
Putative membrane protein	SAR0623	4	10-14	1.6
Putative membrane protein	SAR2189	4	6-15	1.5
General stress protein-like protein	SAR1817	2	8-16
Putative fatty acid oxidation complex protein	SAR0224	1	38-40	1.4
FecCD transport family protein	SAR2266	6	6-14	1.6a
Shikimate-5-dehydrogenase	SAR1673	13	10-16	1.3
Mutants with decreased susceptibility
Amino acid permease	SAR1343	1	20-23	1.4
Putative phosphate transport protein	SAR0674	6	8-16
Putative pyruvate carboxylase	SAR1088	3	9-12	1.2
Serine/threonine protein kinase	SAR1196	3	8-14
Hypothetical protein (dak2)	SAR1202	1	52-54	1.6b
Putative ABC transport system protease (potB)	SAR1075	4	8-14

^aDetermined by using a deletion mutant (Fig. 2).

^bSee Fig. 3.