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Model structures of the scFv fragments c12 B5–6 (A), 4D5-Flu (B), and FITC-E2 (C) with bound fluorescein. Altered amino acid positions are indicated for the scFv fragments c12 B5–6 and FITC-E2 (e.g., His H58 Ala indicates that histidine at position 58 in the heavy chain is mutated to alanine).
