TABLE 1.

Bacterial strains used in this study^a

Species and strain	Description	Reference or source
S. aureus
ATCC 35556 (SA113)	Laboratory strain, biofilm proficient	23; ATCC
NCTC 8325 (NRS-77)	Laboratory strain	36; NARSA
MW2 (NRS-123)	CA-MRSA, USA 400b	1; NARSA
MSSA	Clinical isolate, osteomyelitis	3
MRSA-14234	Clinical isolate, CA-MRSA	University of Massachusetts Medical Center, Worcesterc
MRSA-22733	Clinical isolate, CA-MRSA
MRSA-23542	Clinical isolate, CA-MRSA, USA 300b
MRSA-24102	Clinical isolate, CA-MRSA, USA 300
MRSA-24209	Clinical isolate, CA-MRSA, USA 300
MRSA-27687	Clinical isolate, CA-MRSA, USA 300
MRSA-33548	Clinical isolate, CA-MRSA, USA 300
MRSA-35392	Clinical isolate, CA-MRSA, USA 300
MRSA-38230	Clinical isolate, CA-MRSA, USA 300
MRSA-43349	Clinical isolate, CA-MRSA, USA 300
MRSA-44081	Clinical isolate, CA-MRSA, USA 300
MRSA-47263	Clinical isolate, HA-MRSA, USA 100
MRSA-49404	Clinical isolate, CA-MRSA, USA 300
MRSA-58336	Clinical isolate, HA-MRSA, USA 100b
MRSA-1094	Clinical isolate, HA-MRSA, USA 100
ATCC 35556 (pAJ22)	pAJ22 constitutively expresses β-galactosidase encoded by lacZ	39, this study
SA113 Δica::Tc	PNAG deficient	10
ATCC 35556 ΔatlA-atlR::erm	Deficient in major autolysin	This study
S. epidermidis
18972	Clinical isolate, endocarditis, MRSE	Oscient Pharmaceuticals, Waltham, MA
1457		26
O-47		19
ATCC 35984 (RP62A)		5; ATCC
CoNS
WTBF-40	Clinical isolate	University of Massachusetts Medical Center, Worcester
WTBF-41	Clinical isolate
WTBF-45	Clinical isolate
WTBF-46	Clinical isolate
WTBF-47	Clinical isolate
WTBF-48	Clinical isolate
E. faecalis
ATCC 51299	Clinical isolate, VRE	ATCC
ATCC 51575		ATCC
F317	Clinical isolate, VRE	Northwestern Memorial Hospital, Chicago, ILd
F177	Clinical isolate, VRE
F217	Clinical isolate, VRE
E. faecium
EF208	Clinical isolate, VRE
1401	Clinical isolate, VRE
EF1509	Clinical isolate, VRE
F118	Clinical isolate, VRE
E. gallinarum 2392	Clinical isolate, VRE
E. coli ATCC 25922	Reference strain, biofilm proficient	ATCC
P. aeruginosa PAO1	Laboratory strain, biofilm proficient	51

^aAbbreviations: NARSA, Network on Antibiotic Resistance in Staphylococcus aureus (Chantilly, VA); CA, community acquired; HA, hospital acquired; MRSE, methicillin-resistant S. epidermidis.

^bThe USA 400, USA300, and USA100 designations of MRSA refer to distinct genetic lineages assigned on the basis of pulsed-field gel electrophoresis analyses (31).

^cClinical strains of MRSA and CoNS strains were selected from the collection at the University of Massachusetts Medical Center, Worcester, and kindly provided by Richard Ellison.

^dClinical strains of VRE were selected from the collection at Northwestern Memorial Hospital, Chicago, IL, and were provided by Jason Pryztwoski.