TABLE 4.
Open reading frames identified in pEK204
| Gene name | Nucleotide positiona | Function encoded |
|---|---|---|
| repY | 378-467 | Regulatory protein of RepZ |
| repZ | 455-1486 | Replication protein of the I1 replicon |
| yafA | Compl. 2556-3065 | Hypothetical protein |
| yafB | Compl. 3121-3681 | Hypothetical protein |
| yagA | 3966-5423 | Hypothetical protein |
| tnpA | Compl. 5711-5806 | Transposase of Tn3, truncated |
| tnpA | 5997-7259 | Transposase of ISEcp1 |
| blaCTX-M-3 | 7594-8469 | β-Lactamase CTX-M-3 precursor |
| tnpA | Compl. 8772-11930 | Transposase of Tn3, truncated |
| tnpR | 11899-12492 | Resolvase of Tn3 |
| blaTEM-1 | 12675-13535 | β-Lactamase TEM-1 precursor |
| impB | Compl. 15037-16308 | UV protection and mutation protein |
| impA | Compl. 16308-16745 | UV protection and mutation protein |
| impC | Compl. 16742-16990 | UV protection and mutation protein |
| yccA | 17098-17370 | Hypothetical protein |
| yccB | 17381-18310 | Hypothetical protein |
| ycdA | 18307-18628 | Hypothetical protein |
| parM | 19078-20058 | Plasmid segregation protein ParM |
| stbB | 20055-20483 | Stable plasmid inheritance protein |
| ycdB | 20874-21557 | Putative methylase |
| yceA | 21558-21779 | Hypothetical protein |
| yceB | 21793-22227 | Hypothetical protein |
| ycfA | 22272-23042 | Hypothetical protein |
| ycgB | 23455-23880 | Hypothetical protein |
| ycgC | 23927-24349 | Hypothetical protein |
| ychA | 24401-24703 | Hypothetical protein |
| ssb | 25322-25849 | Single-stranded DNA-binding protein |
| ykfF | 25880-26140 | Hypothetical protein |
| ycjA | 26193-28157 | Hypothetical protein |
| psiB | 28117-28644 | Plasmid SOS inhibition protein B |
| psiA | 28641-29360 | Plasmid SOS inhibition protein A |
| ygaA | 29357-29953 | Hypothetical protein |
| ygbA | 30025-30915 | Hypothetical protein |
| ardA | 30415-30915 | Antirestriction protein |
| ydfA | 31644-32078 | Hypothetical protein |
| ydfB | 32172-32438 | Hypothetical protein |
| BASYS00078 | 32615-33004 | Hypothetical protein |
| ygbB | 33001-33903 | Hypothetical protein |
| ygeA | Compl. 34202-34453 | Hypothetical protein |
| BASYS00079 | Compl. 34487-34798 | Hypothetical protein |
| ydiA | 35032-35880 | Hypothetical protein |
| yggA | Compl. 35966-36301 | Hypothetical protein |
| nikA | 36532-36867 | Relaxosome component protein |
| nikB | 36878-39577 | Relaxase |
| trbC | Compl. 39614-41905 | Hypothetical protein |
| trbB | Compl. 39614-41905 | Hypothetical protein |
| trbA | Compl. 42987-44195 | Hypothetical protein |
| finQ | Compl. 44502-45431 | Hypothetical protein |
| pndC | 45769-46053 | Postsegregation killing system, counter protein for PndA |
| pndA | 45908-46060 | Postsegregation killing protein |
| exc | Compl. 47893-48555 | Surface exclusion protein |
| traY | Compl. 48626-50863 | Integral membrane protein |
| traX | Compl. 50891-51475 | F pilin acetylation |
| traW | Compl. 51504-52706 | F pilus assembly |
| traV | Compl. 52673-53287 | F pilus assembly |
| traU | Compl. 53287-56331 | F pilus assembly |
| traT | Compl. 56421-57221 | Surface exclusion |
| traS | Compl. 57205-57393 | Surface exclusion |
| traR | Compl. 57457-57861 | Hypothetical protein |
| traQ | Compl. 58439-59143 | Conjugal transfer protein |
| traP | Compl. 58439-59143 | Conjugal transfer protein |
| traO | Compl. 59143-60432 | Hypothetical protein |
| traN | Compl. 60435-61418 | Aggregate stability |
| traM | Compl. 661429-62121 | Mating signal |
| traL | Compl. 62118-62465 | F pilus assembly |
| sogL | Compl. 62483-66286 | DNA primase |
| sogS | Compl. 62483-65020 | Regulative protein |
| nuc | Compl. 66340-66894 | EDTA-resistant nuclease |
| traK | Compl. 66906-67196 | F pilus assembly |
| traJ | Compl. 67193-68341 | ATP-binding protein |
| traI | Compl. 68338-69156 | DNA helicase |
| traH | Compl. 69153-69611 | F pilus assembly |
| traG | Compl. 70005-70589 | F pilus assembly |
| traF | Compl. 70649-71851 | F pilus assembly |
| traE | Compl. 71937-72761 | F pilus assembly |
| rci | Compl. 72912-74066 | Shufflon-specific DNA recombinase |
| tnpA | 75447-77060 | Transposase of IS66 |
| pilV | Compl. 77739-79163 | Type IV prepilin cluster |
| pilU | Compl. 79162-79819 | Type IV prepilin cluster, prepilin peptidase |
| pilT | Compl. 79804-80364 | Type IV prepilin cluster |
| pilS | Compl. 80374-80988 | Type IV prepilin cluster, prepilin |
| pilP | Compl. 80988-84132 | Type IV prepilin cluster |
| pilR | Compl. 81005-82103 | Type IV prepilin cluster, integral membrane protein |
| pilQ | Compl. 82115-83669 | TypeIV prepilin cluster, ATP-binding protein |
| pilP | Compl. 83680-84132 | Type IV prepilin cluster |
| pilO | Compl. 84179-85414 | Type IV prepilin cluster |
| pilN | Compl. 85407-87089 | Type IV prepilin cluster, secretin protein |
| pilM | Compl. 87103-87540 | Type IV prepilin cluster |
| pilL | Compl. 87540-88607 | Type IV prepilin cluster, lipoprotein |
| pilK | Compl. 88641-89234 | Type IV prepilin cluster |
| pilJ | Compl. 89231-89527 | Type IV prepilin cluster |
| pilI | Compl. 89694-89948 | Type IV prepilin cluster |
| BASYS00080 | Compl. 90108-90661 | Hypothetical protein |
| traC | Compl. 90834-91517 | F pilus assembly |
| traB | Compl. 91771-92304 | F pilus assembly |
| traA | Compl. 92309-92432 | F pilin subunit |
a
Compl., gene is the reverse complement of the positions shown.