Figure 6. NPC survival and differentiation.
(a) Graph of the total number of NPCs at implantation compared to the total number of NPCs found in the spinal cord at eight weeks. (b) Image demonstrating GFP-positive NPCs in the cord as well as their close association with streptavidin-Texas Red labeled blood vessels (red). (c–k) Confocal images of longitudinal sections in which NPCs were found in the injury epicenter in the implant or in tissue adjacent to the implant. (c–e) GFP-positive NPCs (d) stained positively with nestin (c). (e) Merged image of (c) and (d). (f–h) GFP-positive NPCs (g) expressed GFAP (f). (h) Merged image of (f) and (g). (i–k) Representative image of GFP-positive NPCs (j) expressing ®III tubulin (i). (k) Merged image of (i) and (j). (l) Table of the % of cells (± S.E.M.) in the spinal cord positive for each antibody.
