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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Eur J Neurosci. 2009 Jan;29(1):132–145. doi: 10.1111/j.1460-9568.2008.06567.x

Figure 7. Neurofilament immunostaining and GAP-43.

Figure 7

NF-positive neurons are red and DAPI-labeled cells nuclei are blue. A cross section at the injury epicenter on the unlesioned side from the lesion control (a) and implant plus NPCs groups (b). (c) Graph of the mean area of NF-positive cells on the unlesioned side at the lesion epicenter per 20X field. Error bars = ± SEM. * = p<0.05, ** = p<0.01, and *** = p<0.001. (d) Graph of the area of NF-positive cells at the implant/host interface. * = p<0.05 The implant plus ECs and implant plus ECs:NPCs show significantly more ingrowth than the implant alone group. (e) Schematic of a cross-section showing where the images are from in the tissue. (f) A longitudinal section from an implant plus NPCs cord showing GAP-43 fibers extending from the unlesioned side (right side of dotted line) into the lesion (left side of dotted line). (g) Longitudinal section stained with NF (red) and DAPI (blue). This is the sister section to the longitudinal image in Fig. 2n. GFP-positive NPCs are seen in the gel (white arrow). The red patches in (g) are remnants of the hydrogel that are autofluorescing. Scale bars in (a), (b) and (f) are 50 μm.