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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Int J Cancer. 2009 Oct 1;125(7):1728–1734. doi: 10.1002/ijc.24553

Figure 3.

Figure 3

EGF or IL-6 stimulation induces PIAS3-STAT3 complex binding to STAT3 consensus sequence. After EGF or IL-6 stimulation, nuclear extracts were made from A549 cells. The extracts were mixed with the STAT3 DNA binding consensus sequence hSIE and EMSA performed identifying activated STAT3 in the extracts. STAT3 was verified by incubating STAT3 antibody with the hSIE complex resulting in supershift of the band. PIAS3 was also identified in the complex through supershift of hSIE with a PIAS3 antibody. Arrows indicate supershift with anti-PIAS3 antibody.