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. Author manuscript; available in PMC: 2009 Oct 20.
Published in final edited form as: FEBS Lett. 2008 Jun 20;582(17):2527–2531. doi: 10.1016/j.febslet.2008.06.017

Figure 4. Analysis of zinc inhibition of AP-B by Lineweaver-Burk plot.

Figure 4

Inhibition of AP-B (22 nM) by zinc (at 1, 2, 5, and 10 μM) was assessed by inverse Lineweaver-Burk plots [15], which indicated zinc as a mixed inhibitor. The Lineweaver-Burk plot also showed that AP-B (without zinc) has Km value of 27 μM Arg-MCA and Vmax of 1.2 μM/min. The Ki and Ki′ values for zinc inhibition of enzyme and enzyme/substrate complexes were calculated as 6 μM and 12 μM zinc, respectively.