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. Author manuscript; available in PMC: 2009 Oct 20.
Published in final edited form as: FEBS Lett. 2008 Jun 20;582(17):2527–2531. doi: 10.1016/j.febslet.2008.06.017

Figure 6. Zinc increases susceptibility of AP-B to degradation by trypsin.

Figure 6

Purified AP-B (835 ng) was incubated without (lane 1) or with (lane 2) ZnCl2 (250 μM) (for 5 min.), and was then subjected to incubation with trypsin (50 ng) at 30°C for 40 minutes. The integrity AP-B was then assessed by western blots with anti-AP-B, with equal amounts of AP-B (58 ng) present in each of lanes 1 and 2. Results show that zinc induced the susceptibility of AP-B to degradation by trypsin.