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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Immunol. 2009 Mar 1;182(5):3278–3284. doi: 10.4049/jimmunol.0803417

FIGURE 7.

FIGURE 7

Liver damage is Fas independent. A, Liver CD4+ T cells were isolated from 11-day-old littermate Tgfb1+/− mice (n = 3) and Tgfb1−/− mice (n = 3). mRNA was purified, converted to cDNA, and FasL and β-actin were measured by RT-PCR. “No RT” controls indicate mRNA samples to which reverse transcriptase was not added, showing that the PCR amplicons were not templated by contaminating genomic DNA. B, Plasma AST was measured from 11- to 12-day-old mice. Bars indicate mean + SD. Values of p are shown (n = 4–7 mice per group).