Figure 3.
Golgi membrane tubule formation during reassembly requires both PLA2 activity and microtubules. (A-C) Cells maintained in the continuous presence of ONO produce few tubules. Panels A-C were reproduced from Supplemental Movie 3. (D, E) Cells treated with ONO, but then allowed to recover in the presence of nocodazole. Panels were reproduced from Supplemental movie 4. In this experiment cells were treated with ONO for 45 min, nocodazole (6 μg/ml) was added during the last XX min of ONO incubation, and then cells were washed free of ONO but kept in the continuous presence of nocodazole. (D) Quantitation of Golgi recovery expressed as a Fragmentation Index (100% = 100% of cells with fragmented Golgi complexes; 0% = cells with normal Golgis.