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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Genes Chromosomes Cancer. 2009 Sep;48(9):806–815. doi: 10.1002/gcc.20685

Figure 3.

Figure 3

Cleavage of MLL in TK6 cells detected by LM-PCR. (A) DNA gel after LM-PCR to detect MLL specific fragmentation after exposure to 0.5µg/ml anti-CD95 antibody. Evidence for increased fragmentation ∼300bp is observed that, after Southern blotting with a MLL specific probe (not shown), confirms MLL specificity. DNA from this region extracted from gel, cloned and sequenced to determine precise location of cleavage. (B) Results of cloning and sequencing DNA cleavage sites within the region demonstrated in Fig. 2 and plotted using the same MLL notation. Data plotted in 10bp bins. Shown to scale are sites of Palindromic DNA (Orange) and Topoisomerase II consensus binding site (Green).