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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Neurosci Res. 2009 Mar;87(4):844–856. doi: 10.1002/jnr.21908

Fig. 4.

Fig. 4

Neurite outgrowth from explants seeded on patterned NGF expression. DRG explants were cocultured on patterned NGF expression. Neurites extending toward the pattern of NGF expression exhibited longer lengths compared with neurites extending away from the pattern, with explants cultured 9 hr (a,b) and 24 hr (c,d) after HEK293T seeding. The pattern of expression is to the right of the cultured explant (a,c), and a zoom panel for each explant displays the neurites in detail (b,d). Quantification of neurite length, represented as the ratio of the average neurite length toward vs. away from the pattern of expression, while varying the incubation time between cell seedings (9, 24, and 48 hr) and the distance from the pattern center (e). Values are reported as mean ± SEM. *Statistically different at P < 0.05. Scale bars = 250 μm in a,c; 100 μm in b,d.