Fig. 5.

Neurite orientation from dissociated neurons seeded on patterned NGF expression. Dissociated DRG neurons were cocultured on patterned NGF expression. Neurites initiated away from the pattern of NGF expression exhibited guidance toward the pattern, with DRGs cultured 9 hr (a) and 24 hr (b) after HEK293T seeding (neurons and neurites were stained red with TRITC-labeled secondary antibody). The pattern of expression is to the right of the neuron (a,b). Quantification of neurite orientation, represented as the orientation angle in relation to the pattern while varying the incubation time between cell seedings (9, 24, and 48 hr) and the distance from the pattern center (c). Values are reported as mean ± SEM. *Statistically significantly different at P < 0.05. Scale bars = 50 μm. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]