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. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Cancer Res. 2009 Oct 13;69(20):7986–7993. doi: 10.1158/0008-5472.CAN-09-2266

Figure 4. Glioblastoma cells require GDH to survive glucose deprivation.

Figure 4

A, Cells were transfected with a control siRNA or siRNAs directed against the GLUD1 transcript. The effect on GDH protein abundance (top) and flux (bottom) was determined in the presence and absence of glucose. The average and SD of three independent cultures are shown. B-C, Cells transfected with the control or GLUD1 siRNAs were subjected to glucose withdrawal, and effects on morphology and viability were determined. The metabolites CH3-Pyr and dm-αKG were tested for their ability to rescue viability in glucose-deprived cells. The average and SD of three independent cultures are shown for each condition. D, Glucose-deprived cells expressing a control (NC) shRNA or an shRNA against the GLUD1 transcript (GLUD1-A) were cultured with L-[3-13C]-Gln. Labeling in carbon 4 of glutamate was analyzed by 13C NMR.