A, The effect of EGCG on GDH activity was determined in glucose-deprived cells. B, Glioblastoma cells were cultured in the presence or absence of glucose, EGCG, CH3-Pyr and dm-αKG. The effects of these treatments, alone or in combination, were determined on cell viability at 8 and 16 hours. C, Glioblastoma cells were cultured in the presence or absence of EGCG, an Akt inhibitor or 2-DG, alone or in combination. After 24 hours, viability was determined by Trypan blue staining. In all experiments, the average and SD of three independent cultures are shown for each condition. *: p≤0.005.