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. 2009 Oct 9;5(7):637–646. doi: 10.7150/ijbs.5.637

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Native polyacrylamide gel picture of native HDL and reconstituted particles. To prepare synthetic particles, POPC, TG and CE were mixed proportionally and dried to completion under argon gas. A protein component, apoHDL, apoA-I or D-4F in buffer was then added to the pellet. The sample was sonicated with a microtip sonicator to create the synthetic particles. 20µl of each sample was loaded onto a native acrylamide gel. After running at 90V for 2 hours, the gels were stained with Coomassie blue. Lane 1, marker; Lane 2, synthetic D-4F particles; Lane 3, synthetic apoA-I particles; Lane 4, reconstituted apoHDL (rHDL) particles; Lane 5, native HDL (nHDL).