Fig. 3.

Effects of n-3 PUFAs on sterol-regulatory element binding protein cleavage-activating protein (SCAP), Insig-1, and Insig-2 expression. A: Primary rat hepatocytes were treated with and without insulin and 20:5,n-3 or 22:6,n-3 for 24 h. After treatment, microsomal protein was isolated for analysis of SCAP protein by immunoblotting. The level of SCAP protein was quantified, and the results are shown as percentage of control and are representative of two separate studies. Veh, vehicle. B, C: Total RNA was extracted from primary hepatocytes treated as described for Fig. 2C. Levels of Insig-1 (B), Insig-2 (C), and cyclophilin mRNA were measured by real-time PCR. Results are presented as fold change in Insig/cyclophilin and are representative of three separate studies with triplicate samples/treatment (mean ± SD). Solid line-circles, insulin-treated cells; dashed line-squares, 22:6,n-3-treated cells; dotted line-triangles, insulin- and 22:6,n-3-treated cells.