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. Author manuscript; available in PMC: 2009 Oct 20.
Published in final edited form as: J Lipid Res. 2006 Jun 21;47(9):2028–2041. doi: 10.1194/jlr.M600177-JLR200

Fig. 3.

Fig. 3

Effect of insulin and liver X receptor (LXR) agonist on fatty acid elongase and desaturase expression in rat primary hepatocytes. Rat primary hepatocytes were maintained in Williams E medium containing 10 mM lactate and 10 nM dexamethasone with no insulin overnight. The next day, cells were switched to Williams E medium containing 25 mM glucose and 10 nM dexamethasone [vehicle (Veh)] in the presence or absence of insulin (Ins; 1 μM) or the LXR agonist T0901317 (T1317; Cayman Chemical Co., Ann Arbor, MI) (1 μM). After 24 h of treatment, cells were harvested for RNA extraction and the measurement of nuclear sterol-regulatory element binding protein-1 (SREBP-1) and SREBP-2 by immunoblot analysis (inset; duplicate samples/treatment). Elongase and desaturase mRNA abundance was quantified by qRT-PCR. Results are expressed as fold change in mRNA versus vehicle (transcript/ cyclophilin) (means ± SD; n = 4). Results are representative of two separate studies. * P ≤ 0.05 versus vehicle by ANOVA.