Table 2.

Verification of in vivo identified cells with in situ markers

in vivo identification:	Total number		Ratio of cells in situ/in vivo
in vivo identification:	cells	embryos	lcp	hbbe2	mpx
macrophage	99	19	90/91^a	not done	0/21
erythrocyte	590	24	0/160	571/590^b	0/344
neutrophil	39	12	0/11	0/34	26/28^c
thrombocyte ^d	55	10	not done	0/55	0/52

Clones were examined at 24 hours and again between 30 to 36 hours before being fixed and processed for in situ hybridization. In general, but not always ventral clones were examined with two different riboprobes as detailed in the methods section.

Of 91 labeled cells identified as macrophages, 90 cells expressed the marker lcp which is specific for macrophages and possibly other white blood cells.

The marker hbbe2 is specific to erthrocytes.

The marker mpx is specific for neutrophils.

Thrombocytes do not express the thrombocyte specific marker cd41 until 48 h.