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Morphological Features of A549 Cells following Selected DUB Knockdown
Twelve deubiquitinating enzymes (DUBs) identified in our screen as required for HGF-mediated scattering of A549 cells were knocked down with pooled oligonucleotides from a siGenome library. One set of cells was treated with 50 ng/ml HGF for 12 hr (+HGF panels), while the other set was left untreated (−HGF panels). Cells were stained with crystal violet. Scale bars represent 50 μm.
