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. 2009 Sep 15;19(17):1463–1466. doi: 10.1016/j.cub.2009.07.040

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© 2009 ELL & Excerpta Medica.

Open Access under CC BY 3.0 license

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Correlation of USP33 and USP30 Knockdown Efficiency with HGF-Induced Scattering Response in A549 Cells

(A) Estimation by western blotting of endogenous protein of the knockdown efficiency for eight individual siRNA oligonucleotides directed against USP33 (top) and their respective effects on HGF-mediated cell scattering of A549 cells (bottom) (IS, inhibition of scattering; S, scattering).

(B) No antibody is currently available for USP30, but each of the four individual oligonucleotides effectively represses transient expression of USP30-GFP in HeLa cells as judged by western blotting with GFP-antibody, as well as inhibiting HGF-mediated scattering of A549 cells (IS).