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DUB Requirements for HGF-Dependent Wound Healing in A549 Cells
Confluent monolayers of A549 cells were pretreated for 72 hr with siRNA oligonucleotides (40 nM) consisting of pools from the siGenome DUB library targeting specific DUBs. After introducing a scratch, cells were incubated in full medium supplemented with 50 ng/ml HGF and visualized 20 hr later.
