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. Author manuscript; available in PMC: 2009 Oct 20.
Published in final edited form as: Kidney Int. 2009 Jan 21;75(8):800–808. doi: 10.1038/ki.2008.690

Figure 4. Analysis of signaling protein phosphorylation in ventricular tissue obtained from surgically induced renal injury (SIRI) or sham mice.

Figure 4

(a) Analysis of cardiac signaling protein phosphorylation in ventricular tissue obtained 8 weeks after SIRI (n=5) or sham surgery (n=4). Ventricular tissue was homogenized, protein lysates generated, and proteins were separated by SDS-PAGE followed by immunoblotting with primary antibodies directed against phosphorylated-Akt1/2 (p-Akt), phosphorylated-ERK1/2 (p-ERK1/2), phosphorylated-ribosomal S6 protein (p-S6), phosphorylated-4E-BP1, and α-actin as a loading control. (b) Scanned blot densitometry was performed with Image J (v1.24) software. *P<0.05 vs sham.