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Samples were in 10 mM sodium phosphate, pH 7.0. βB1, βB1ΔN56, and βB1ΔN47 are indicated by black solid, dashed, and dotted lines respectively. βB1 and βB1ΔN56 unfolded with 8M urea are indicted by gray solid and, dashed lines, respectively. Full far-UV spectra of unfolded proteins are not shown because the absorption by 8M urea precludes measurements < 210 nm (54).
