Figure 2.

Detection of Mycoplasma (M.) dilutions by Venor®GeM (A) and McCT (B). Both assays were performed using 6-fold dilution series of one cell lysate positive for M. arginini and M. hyorhinis. (A) PCR products from the commercial Mycoplasma kit were loaded on a 2% agarose gel showing the internal PCR control (191 bp) and a Mycoplasma-specific band (267 bp). (B) Net MFI values obtained after hybridization of the McCT PCR products to Mycoplasma species-specific probes are shown. The cut-off is indicated by the dotted line.