Table 4.
Specificity of McCT PCR product detection: viral templates
| PCR template | Virus-specific probe |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SMRV |
HPV18 | HSV1 | HSV2 | VZV | EBV | CMV | HHV6 | HHV7 | HHV8 | HBV | Adeno virus 5 | VP1 | SV40 VP3 | T-antigen | ||
| env | gag | |||||||||||||||
| SMRV | 1687a | 488 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
| HPV18 | 0 | 0 | 299 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| HSV1 | 0 | 0 | 1 | 1116 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| HSV2 | 0 | 0 | 1 | 1 | 350 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| VZV | 1 | 0 | 0 | 0 | 0 | 259 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| EBV | 0 | 1 | 0 | 0 | 0 | 0 | 122 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| CMV | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 429 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 |
| HHV6 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 254 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
| HHV7 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1732 | 0 | 1 | 0 | 1 | 0 | 1 |
| HHV8 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 355 | 0 | 0 | 0 | 1 | 0 |
| HBV | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2660 | 0 | 0 | 0 | 0 |
| Adeno virus 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1585 | 1 | 0 | 1 |
| SV40 VP3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 210 | 1 | 0 |
| SV40 VP1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 450 | 0 |
| SV40 T-antigen | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1068 |
aEach line represents hybridization results of the multiplex PCR product of one template hybridized to a mixture of 45 distinct probes; results of only 16 probes are shown, no positive signals were observed with any of the 29 other probes; numbers are net MFI values computed as described in ‘Materials and Methods’ section, values >5 are considered positive.