Table 5.

Specificity of McCT PCR product detection: mammalian species-specific probes

PCR template	Species-specific probe/gene region
	Human		Mouse		Rat		Monkey		Chinese hamster	Dog	Cat	Guinea pig	Rabbit	Quality control
	β-globin	TSPYL1	β-globin	16S	β-globin	16S	β-globin	16S	16S	16S	16S	16S	16S	PolA
Human	701a	189	0	1	0	0	0	0	0	0	0	0	0	324
Mouse	0	3	68	739	0	0	0	0	0	0	0	0	0	229
Rat	0	0	0	0	496	62	0	0	0	0	0	0	0	231
Green monkey	2	14b	0	1	0	0	310	374	0	0	0	0	0	147
Chinese hamster	0	0	0	0	0	0	0	0	672	0	0	0	0	207
Dog	0	0	0	0	1	2	1	0	1	1166	2	0	2	182
Cat	1	1	0	0	0	0	0	0	0	2	866	1	0	174
Guinea pig	0	0	0	0	0	1	0	0	1	0	0	1088	0	220
Rabbit	0	0	0	0	0	0	0	0	1	0	0	0	155	173
H2O-PCR	0	0	0	0	1	0	0	0	0	0	0	0	1	0

^aEach line represents hybridization results of the multiplex PCR product of one template hybridized to a mixture of 45 distinct probes; results of only 14 probes are shown, no positive signals were observed with any of the 31 other probes; numbers are net MFI values computed as described in ‘Materials and Methods’ section, values >5 are considered positive.

^bWeak cross-reactivity.