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. 2009 Aug 19;37(18):5981–5992. doi: 10.1093/nar/gkp658

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© The Author 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses?by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — PspF_1–275 F85Y slowly activates transcription from the supercoiled (sc) nifH promoter. (A) Full-length (FL) transcription from the nifH promoter as a function of increased activation time in the presence of PspF_1–275 WT and PspF_1–275 F85Y (PspF_1–275 F85A acts as a negative control). The relative intensity of each transcript is represented graphically below the gel. (B) Abortive transcription as a function of increased activation time in the presence of PspF_1–275 WT and PspF_1–275 F85Y (PspF_1–275 F85A acts as a negative control). RPo formation is directly correlated with the amount of abortive product (UpGpGpG) generated. All the reactions were conducted at the same time, run on the same gel and scanned with the same contrast. The lanes were rearranged for clarity. The experiment was minimally performed (independently) in triplicate and the results obtained similar.