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. 2009 Aug 19;37(18):5981–5992. doi: 10.1093/nar/gkp658

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© The Author 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses?by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Gel filtration profiles of the F85 variants at room temperature. (A) The elution profiles of the F85 variants chromatographed in the absence of nucleotide. Two concentrations were used for each F85 variant, low (20 μM light grey line) and high (63 μM black line). (B) The elution profiles of the F85 variants chromatographed in the presence of 0.4 mM ADP (using the lower 20 μM protein concentration). Standard globular proteins were used for calibration: thyroglobulin (669 kDa), apoferritin (443 kDa), β-amylase (200 kDa), bovine serum albumin (66 kDa) and carbonic anhydrase (29 kDa). Absorption unit (AU) on the scale corresponds to an A₂₈₀ of 1.