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F85 potentially senses the DNA conformation at the −12 fork junction DNA structure. ‘Trapping’ reactions were conducted on different 32P-labelled linear DNA probes which represent the conformation of promoter DNA at different steps en route to the RPo. A–B: assays with σ54WT, C–D: assays with σ54G4L. The stable trapped complexes formed were analysed by native-PAGE and detected by PhosphorImager.
