Figure 7.

Effects of various inhibitors on the (a) uptake and (b) gene expression of MCEC transfected with F complexes (N/P 20). Cells were pre-incubated with inhibitors for 1 h prior to exposure to F complexes containing Cy5-labelled DNA (for uptake quantification using flow cytometry) or unlabelled DNA (for gene expression). Values were all referenced to control cells normally transfected with F complexes without any inhibitors (assigned as 100%). In cases whereby the inhibitors had to be dissolved in DMSO, control cells were also transfected in the presence of the same volume of pure DMSO. Error bars represent SD.