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. 2009 Aug 19;37(18):6276–6289. doi: 10.1093/nar/gkp651

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© The Author 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses?by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — FRET study. Confocal images taken of live MCEC at row (a) 4.5 h, row (b) 8.5 h and row (c) 26 h after initial exposure to peptide F/DNA complexes at N/P 20. The DNA had been double-labelled with both Cy5 and Cy3. During imaging, only Cy3 was excited. If there was FRET, Cy5 (false-coloured red) can also be detected, which will then co-localize with Cy3 (green) to give yellow signals in the merged image. Row (d) A nucleus at 26 h showing large scale accumulation of uncondensed DNA. All cells were stained for their nuclei with Hoechst (blue) and presented with the bright field (BF) images.