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Optimizing RNA display in Y3H. (A) Predicted secondary structure of original MS2 and T-cassette transcripts alone (RNAs 1 and 2), with 31-nt anti-p50 (RNAs 3 and 4), and with R1 (RNAs 5 and 6) for RNA display in Y3H. (B) Northern blot comparison of T-cassette and MS2 RNA transcript accumulation in test strains discussed in Table 1. Note that the highly-structured T-cassette RNAs resist complete denaturation, causing retarded mobility (*).
