Figure 5.

Y3H selection of anti-p65 RNA aptamers from early SELEX rounds. (A) Percent RNA recovered during original SELEX that yielded the anti-p65 RNA aptamer R1 (25). (B) Candidate anti-p65 RNA aptamers from SELEX round 6. (C) Predicted secondary structures of Y1, Y3, and truncation variants. The 5′ terminal nt of each RNA is circled. (D) Electrophoretic gel mobility shift binding isotherms for murine p65₂ in the presence of R1 (closed circles), Y1 (closed squares), Y3 (open squares), truncation Y1-1 (open circles), or truncation Y1-1T with substituted tetraloop (plus).