Figure 6.

Y1 and Y1-1 RNA aptamers as decoys for NF-κB. Labeled κB DNA (1 nM) was incubated alone (lanes 1 and 15) or in the presence of p65₂ (20 nM) alone (lane 2) or together with various unlabeled RNA competitors (lanes 3–14): R1 RNA (lanes 3–5), anti-p50 RNA (lanes 6–8), Y1 RNA (lanes 9–11), and truncated Y1-1 (lanes 12–14), Triangles indicate increasing unlabeled molar excess competitor concentration [3-, 6- or 12-fold competitor (FC; lanes 3–5)] and [25-, 50- or 75-fold competitor (lanes 6–14)] relative to p65₂ concentration.