Figure 4.

Consequences of UNG2 mutations on inhibition of LTR-driven transcription. (A) 293T cells were transfected with equivalent amounts of pcDNA-UNG2-GFP or pcDNA-UNG2_QD153-154LE (QD) or left untransfected (Mock). Expression of UNG2 constructs in the corresponding cell extracts was controlled using anti-UNG2 immunoblots (upper panel). Actin immunoblots were used as protein loading controls (lower panel). (B) 293T cells (8 × 10⁴) were cotransfected with 0.2 µg of HIV_LTR-Luc and 0.1 µg of pCMV-Tat plasmids or with 0.2 µg pcDNA-UNG2-GFP or 0.2 µg pcDNA-UNG2_QD153-154LE (QD). Two days after transfection, cells were analyzed in triplicate for luciferase expression used as an index of HIV-1 LTR activity. Data are represented as the fold of LTR promoter activity observed in absence of Tat expression.