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. 2009 Aug 20;37(18):6008–6018. doi: 10.1093/nar/gkp673

Figure 5.

Figure 5.

Consequences of UNG2 overexpression on PMA- and TNF-α-induced HIV-1 LTR transactivation. Hela LTRHIV-1-Luc cells were transfected with 0.8 µg of pGFP or pUNG2-GFP and cultured for 24 h in medium alone (CTL), or supplemented with PMA (20 ng/ml) or TNF-α (50 ng/ml). Subsequently, cell extracts were analyzed for content in luciferase activity. Experiment was carried out in triplicate and results are represented as the fold of activation observed in the pGFP control condition normalized to one. As a control, some Hela LTRHIV-1-Luc cells were co-transfected with 0.1 µg pCMV-Tat and 8 µg of pGFP.